Yes,
it does matter. I tune for smoothest running to 3000 rpm. (Yes, I do freely stipulate the dark force has run strong through my past life and whispers to me often.)
If you are seeing scatter,(a wavering of the timing), there is a reason or several. For me, it was a slack timing chain and tensioner that passed the normal tests for those components ,yet; had a related effect. Find the source.
My timing is steady. Yours should be as well.